性控分离精子凋亡程度对牛体外受精的影响

doi:10.11843/j.issn.0366-6964.2014.06.006

Abstract

Abstract:

The present study utilized three staining assays (Annexin-V，mitochondrial membrane potential (JC-1)，and TUNEL) for flow cytometric analysis of apoptosis in sex-sorted sperm from four different bulls (A，B，C and D).Correlations between sperm quality and IVF efficiency were then assessed to determine which assay provided the best prediction of IVF efficiency.The results showed that: (1) the percentage of viable sperm (AN^-•PI^-) in X-sorted semen from bull A (89.30%±3.04%) was significantly higher than that from other bulls ((74.39%±3.24%)-(81.17%±4.25%); P<0.05)，while the percentage of DNA fragmentation sperm from bull A (12.24%±1.25%) was significantly lower than that from other bulls ((19.09%±1.27%)-(27.56%±3.17%); P<0.05); (2) the percentage of cells reaching the cleavage and blastocyst stages after in vitro fertilization (IVF) in sex-sorted sperm from bull A（（76.82%±3.00%），（20.45%±1.44%）） were significantly greater than those from the other bulls ((22.00%±6.85%)-(31.88%±6.69%)，(3.33%±2.67%)-(4.00%±3.26%); P<0.05); (3) a significant positive correlation was observed between viable sperm and the percentage of cells at the cleavage or blastocyst stages (P<0.05) and a negative correlation was found between early apoptotic sperm and the percentage of cells at the cleavage or blastocyst stages (P<0.05).In conclusion，these results indicated that the Annexin-V assay was the most reliable technique for the prediction of the IVF success of sex-sorted bovine sperm.

CLC Number:

S823.3

HAO Hai-sheng，REN Jing-jing，ZHAO Xue-ming，WANG Ling-ling，DU Wei-hua，WANG Chang-fa，ZHU Hua-bin. Effects of the Degree of Apoptosis in Sexed Sperms on Bovine Fertilization in vitro[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(6): 893-900.

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Effects of the Degree of Apoptosis in Sexed Sperms on Bovine Fertilization in vitro

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